In vitro experiments
In vitro cultivation of gut microbiomes
In vitro cultivation of gut microbiomes
- Stability reached after 48h
- >90% of OTUs retained
- >90% of genera with >1% abundance retained
- stable for 120h (and beyond)
- highly reproducible results
- Total quality control (LIMS)
- Full control of temperature, pH, oxygen, cell growth
- Fully anaerobic workflow
in vitro gut barrier model
Measurement of transepithelia resistance with a Voltohmmeter and a Ussing chamber and morphological assessment of cell-cell connections
- Caco2 based model
- TEER (Voltohmmeter and Ussing chamber)
- Transwell cultures
- variety of readouts: cytokine production in supernatant, cellular gene and protein expression, visualization with immunofluorescence
- assessment of ”tortuosity” (AI based)
in vitro innate immune model
Induction of ROS production in human neutrophils in response to probiotic strains alone (left) and pathogens of interest + probiotic strains (right)
- Human neutrophils
- Large set of readouts
- ROS production
- phagocytosis
- chemotaxis
- NET formation
- protein expression (e.g. calprotectin)
CBmed GmbH Center for Biomarker Research in Medicine
Stiftingtalstrasse 5 8010 Graz Austria office@cbmed.at +43 316 385 28801
