In vitro experiments

In vitro cultivation of gut microbiomes

  • Stability reached after 48h
  • >90% of OTUs retained
  • >90% of genera with >1% abundance retained
  • stable for 120h (and beyond)
  • highly reproducible results
  • Total quality control (LIMS)
  • Full control of temperature, pH, oxygen, cell growth
  • Fully anaerobic workflow

in vitro gut barrier model

Measurement of transepithelia resistance with a Voltohmmeter and a Ussing chamber and morphological assessment of cell-cell connections

  • Caco2 based model
  • TEER (Voltohmmeter and Ussing chamber)
  • Transwell cultures
  • variety of readouts: cytokine production in supernatant, cellular gene and protein expression, visualization with immunofluorescence
  • assessment of ”tortuosity” (AI based)

in vitro innate immune model

Induction of ROS production in human neutrophils in response to probiotic strains alone (left) and  pathogens of interest + probiotic strains (right)

  • Human neutrophils
  • Large set of readouts
  • ROS production
  • phagocytosis
  • chemotaxis
  • NET formation
  • protein expression (e.g. calprotectin)

The COMET Center CBmed is funded within COMET – Competence Centers for Excellent Technologies by the Federal Ministry of Climate Action, Environment, Energy, Mobility, Innovation and Technology (BMK), the Federal Ministry for Digital and Economic Affairs (BMDW), Land Steiermark (Styrian Business Promotion Agency – SFG) and Land Wien (Vienna Business Agency – WAW). The COMET program is executed by the Austrian Research Promotion Agency (FFG).

CBmed GmbH Center for Biomarker Research in Medicine
Stiftingtalstrasse 5   8010 Graz   Austria   +43 316 385 28801